Journal: Cardiology Research and Practice

Article Title: Elevated GTP Cyclohydrolase I Pathway in Endothelial Progenitor Cells of Overweight Premenopausal Women

doi: 10.1155/2020/5914916

Figure Lengend Snippet: The GTPCH I/BH4 pathway and the phosphorylation of eNOS in circulating EPCs in the four groups. The level of GTPCH I (a) or intracellular BH4 (b) of EPCs in normal-weight and overweight premenopausal women was higher than that in normal-weight and overweight men. There was no difference in the level of GTPCH I (a) or intracellular BH4 (b) of EPCs between normal-weight and overweight premenopausal women. No significant difference was found in either eNOS phosphorylation (c) or eNOS protein expression (d) of EPCs between the four groups. (e) Representative photographs of GTPCH I, phosphorylated eNOS and eNOS expression of EPCs. Data are given as mean ± SD. ∗ P < 0.05 vs. the same gender group of normal weight; # P < 0.05 vs. the same weight class of premenopausal women.

Article Snippet: Rabbit antiphosphorylated eNOS and anti-eNOS (1 : 1000; Cell Signaling Technology Inc.) and anti-GTPCH I and β -actin (1 : 1000; Santa Cruz Biotechnology Inc.) were used to evaluate eNOS and GTPCH I expression as previously described [ , , ].

Techniques: Expressing

Journal: International Journal of Environmental Research and Public Health

Article Title: The Role of the Activation of the TRPV1 Receptor and of Nitric Oxide in Changes in Endothelial and Cardiac Function and Biomarker Levels in Hypertensive Rats

doi: 10.3390/ijerph16193576

Figure Lengend Snippet: Expression of guanosine triphosphate cyclohydrolase 1 (GTPCH-1), endothelial nitric oxide synthase (eNOS), protein kinase B (AKT) in ventricular tissue from normotensive and hypertensive rats (black and white bars respectively) with the different treatments. Panel ( A ): GTPCH-1 expression in ventricular tissue ( n = 3–4 rats/group); a = p < 0.05 vs. Normotensive (N), b = p < 0.05 vs. Hypertensive (H), c = p < 0.05 vs. Hypertensive + Capsaicin (H + CS). Panel ( B ): eNOS expression in left ventricle ( n = 3–4 rats/group); a = p < 0.05 vs. Normotensive (N), b = p < 0.05 vs. Hypertensive (H). Panel ( C ): AKT expression in left ventricle ( n = 3–4 rats/group). a = p < 0.05 vs. Normotensive (N), b = p < 0.05 vs. Hypertensive (H), c = p < 0.05 vs. Hypertensive + Capsaicin (H + CS), d = p < 0.05 vs. Normotensive + Capsaicin (N + CS). z = Representative immunoblot. By Duncan’s post hoc test. Data are presented as means ± SEM.

Article Snippet: The membranes obtained were incubated with primary antibodies anti- TRPV1 (V2764) (from Sigma-Aldrich, St. Louis, MO, USA), anti-NOS3 (sc-376751) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-AKT and anti-GTPCH-1 (Calbiochem, La Jolla, CA, USA) overnight at 4 °C.

Techniques: Expressing, Western Blot